Description
ThekitiscomposedofonevialofPEGylateddoxorubicinliposomesandonevialofPEGylatedcontrolliposomes. PEGylateddoxorubicinliposomeformulationissimilar tocommercialDoxil®insize,lipidcompositionandtheamountofencapsulateddrug.Theformulationisforresearchpurposesonlyandforinjectiontolaboratoryanimals.DoxorubicindrugisalreadyloadedintotheliposomesusingremoteloADIngbyammoniumsulfategradient.Theformulationisreadyforinjection. ThecontrolformulationofPEGylateddoxorubicinissimilartoPEGylateddoxorubicininsizeandlipidcomposition,butitdoesnotcontaindoxorubicindrug.Theformulationismadeinammoniumsulfategradient.
Surfacereactiveliposomeformulationssuitableforconjugationtovariousantibodies,proteins,peptidesandligandsarealsoavailable.Formoreinformationaboutsurfacereactiveliposomes(Immunsome®)seehere.
Formoreinformationaboutsurfacereactivedoxorubicinliposomes(Immundox®)suitableforconjugationseehere.
FormulationInformation
StandardDoxosome™Kit-DoxorubicinLiposomes(PEGylated)
| 2-VialKitforStandardDoxosome™(PEGylated) | Specification |
|---|---|
| Vial1 | PreformedliposomescomposedofHSPC:Cholesterol:DSPE-PEG(2000)(57:38:5molarratio) |
| Vial2 | PreformedliposomescomposedofHSPC:Cholesterol:DSPE-PEG(2000)(57:38:5molarratio) |
| LipidCompositionforVial1 | Concentration(mg/ml) | Concentration(mM) | MolarRatioPercentage |
|---|---|---|---|
| Total | 15.96mg/ml | 21.62mM | 100 |
| HydrogenatedSoyPC | 9.58 | 12.22 | 57 |
| Cholesterol | 3.19 | 1.14 | 38 |
| DSPE-PEG(2000) | 3.19 | 8.26 | 5 |
| Buffers,LiposomeSizeandEncapsulatedDrugConcentrationforVial1 | Specification |
|---|---|
| InsideBuffer | AmmoniumSulfate |
| OutsideBuffer | Histidine/SucroseBuffer |
| pH | 6.5 |
| LiposomeSize | 80-100nm |
| EncapsulatedDoxorubicin | 2mg/ml(3.45mM) |
| LipidCompositionforVial2 | Concentration(mg/ml) | Concentration(mM) | MolarRatioPercentage |
|---|---|---|---|
| Total | 15.96mg/ml | 21.62mM | 100 |
| HydrogenatedSoyPC | 9.58 | 12.22 | 57 |
| Cholesterol | 3.19 | 1.14 | 38 |
| DSPE-PEG(2000) | 3.19 | 8.26 | 5 |
| BuffersandLiposomeSizeforVial2 | Specification |
|---|---|
| InsideBuffer | AmmoniumSulfate |
| OutsideBuffer | Histidine/SucroseBuffer |
| pH | 6.5 |
| LiposomeSize | 80-100nm |
LiposomeParticleCalculator
Doxorubicinliposomesareunilamellarandsizedto100nm.Themolarconcentrationofliposomeis21.62mM.Byhaving liposomediameter(nm)andlipidconcentration(µM),youcancalculatethetotalnumberofthelipidsinoneliposomeandthenumberoftheliposomesinonemilliliteroftheliposomesolution.Tousethecalculatorclick here.
Appearance
Doxosome™isared,andDoxosome™Controlisawhitetranslucentliquidbothmadeofnanosizeunilamellarliposomes.Usually,duetothesmallsizeofliposomes,nosettlingwilloccurinthebottomofthevial.Theliposomesarepackagedinambervials.
EducationalVideo
Ordering/ShippingInformation
- Allliposomebasedformulationsareshippedonblueiceat4°Cininsulatedpackagesusingovernightshippingorinternationalexpressshipping.
- LiposomesshouldNEVERbefrozen.Icecrystalsthatforminthelipidmembranecanrupturethemembrane,changethesizeoftheliposomesandcausetheencapsulateddrugtoleakout.Liposomesinliquidformshouldalwaysbekeptintherefrigerator.
- ClientswhoorderfromoutsideoftheUnitedStatesofAmericaareresponsIBLefortheirgovernmentimporttaxesandcustomspaperwork.EncapsulaNanoSciencesisNOTresponsibleforimportationfeestocountriesoutsideoftheUnitedStatesofAmerica.
- WestronglyencouragetheclientsinJapan,Korea,TaiwanandChinatoorderviaadistributor.Toughcustomsclearanceregulationsinthesecountrieswillcausedelayincustomclearanceoftheseperishableformulationsifordereddirectlythroughus.Distributorscaneasilyclearthepackagesfromcustoms.Toseethelistofthedistributorsclickhere.
- ClientsorderingfromuniversitiesandresearchinstitutesinAustraliashouldkeepinmindthattheliposomeformulationsaremadefromsyntheticmaterialandtheformulationsdonotrequirea“permittoimportquarantinematerial”.LiposomesareNOTBIOLOGicalproducts.
- Ifyouwouldlikeyourinstitute’sFedExorDHLaccounttobechargedforshipping,thenpleaseprovidetheaccountnumberatthetimeofordering.
- EncapsulaNanoScienceshasnocontroloverdelaysduetoinclementweatherorcustomsclearancedelays.YouwillreceiveaFedExorDHLtrackingnumberonceyourorderisconfirmed.ContactFedExorDHLinadvanceandmakesurethatthepaperworkforcustomsisdoneontime. AllsubsequentshippinginquiriesshouldbedirectedtoFederalExpressorDHL.
StorageandShelfLife
Storage
Doxosome™productsshouldalwaysbestoredatinthedarkat4°C,exceptwhenbroughttoroomtemperatureforbriefperiodspriortoanimaldosing.DONOTFREEZE.IfthesUSPensionisfrozen,theencapsulateddrugcanbereleasedfromtheliposomesthuslimitingitseffectiveness.Inaddition,thesizeoftheliposomeswillalsochangeuponfreezingandthawing.
ShelfLife
Doxosome™ismadeondailybasis.Thebatchthatisshippedismanufacturedonthesameday.Itisadvisedtousetheproductswithin4monthsofthemanufacturingdate.
Referencesandbackgroundreading
1.HermansonGT.Bioconjugatetechniques.Academicpress;2013Jul25.
2.TorchilinV,WeissigV,editors.Liposomes:apracticalapproach.OxfordUniversityPress;2003Jun5.
3.GrabarekZ,GergelyJ.Zero-lengthcrosslinkingprocedurewiththeuseofactiveesters.Analyticalbiochemistry.1990Feb15;185(1):131-5.
4.YanL,CraytonSH,ThawaniJP,AmirshaghaghiA,TsourkasA,ChengZ.ApH‐ResponsiveDrug‐DeliveryPlatformBasedonGlycolChitosan–CoatedLiposomes.Small.2015Oct1;11(37):4870-4.
5. Silva-LópezEI,EdensLE,BardenAO,KellerDJ,BrozikJA.ConditionsforliposomeadsorptionandbilayerformationonBSApassivatedsolidsupports.Chemistryandphysicsoflipids.2014Oct31;183:91-9.
6.HazraM,SinghSK,andRayS.SurfaceModificationofLiposomalVaccinesbyPeptideConjugation.JournalofPharmaSciTech,2011;1(1):41-47.
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